Currently, there is no treatment to reverse this disease. The mortality of acute TAD is 1%-2% per hour during the first day, while almost half of all patients die by 1 week, and the 1-year mortality is 90% 6. Dissection will expand rapidly leading to serious complications, such as rupture or organ ischemia. In most cases, the dissection develops without aneurysmal degeneration and clinical manifestation 5, so the TAD cannot be detected in a regular physical examination. TAD is defined as disruption of the medial layer, and is caused by intramural bleeding leading to separation of the aortic wall layers followed by formation of a true lumen and a false lumen. The incidence of TAD has increased by 5% per year in recent years, due to increased prevalence of hypertension 4. Epidemiological studies suggest an incidence of acute aortic dissection of about 7-9 cases per 100 000 people per year 3. Thoracic aortic dissection (TAD) is an aggressive and life-threatening vascular disease 1, 2. Thus, CDR-enhanced MRI proposes a potential method for dissection screening, and for monitoring disease progression and therapeutic response. Moreover, NSE was negatively correlated with the time of dissection rupture after BAPN administration (r 2 = 0.8482).Ĭonclusion: As confirmed by in vivo studies, the CDR can identify the exposed Col-IV in degenerated aorta to monitor the progress of aortic dissection from the early stage to the rupture via MRI. The ability to detect rupture of dissected aorta was indicated by a strong normalized signal enhancement (NSE) in vivo. The CDR signal was co-detected by magnetic resonance imaging (MRI) and bioluminescence imaging as early as 2 weeks after BAPN administration (pre-dissection stage).

CDR bound specifically to Col-IV-expressing vascular smooth muscle cells and BAPN-induced dissected aorta. Results: The localization of Col-IV (under the intima) was observed by histology images. We then examined the sensitivity and specificity of CDR at different stages of TAD via MRI and bioluminescence imaging in vivo. Flow cytometry analysis was used to evaluate the cell viability of SMC treated with CDR and fluorescence assays were used to assess the Col-IV targeting ability of CDR in vitro.

Methods: Col-IV-targeted magnetic resonance/ fluorescence dual probe (Col-IV-DOTA-Gd-rhodamine B CDR) was synthesized by amide reaction and coordination reaction. So, we want to build a noninvasive diagnostic method to detect early dissection by identifying the exposed Col-IV via MRI. Type IV collagen (Col-IV) is a major component of the sub-endothelial basement membrane, which is initially exposed followed by endothelial injury as early-stage event of TAD. Thoracic aortic dissection (TAD) is an aggressive and life-threatening vascular disease and there is no effective means of early diagnosis of dissection.

Patterson cdr dicom reconnect database